منابع مشابه
Actin in Xenopus oocytes
It has been found that a high-speed supernatant fraction from Xenopus oocytes extracted in the cold will form a clear, solid gel upon warming. Gel formation occurs within 60 min at 18 degrees-40 degrees C, and is, at least initially, temperature reversible. Gelation is strictly dependent upon the addition of sucrose to the extraction medium. When isolated in the presence of ATP, the gel consist...
متن کاملActin in xenopus oocytes: II. intracellular distribution and polymerizability
The largest oocytes of Xenopus Laevis were broken open in the absence of shearing forces which might transfer actin from particulate to supernatant fractions. Particulate and postmitochondrial supernatant fractions were prepared by centrifugation. SDS-electrophoretic fractionation on polyacrylamide gels and quantitative scanning techniques were used to separate actin and to assay its amount in ...
متن کاملXenopus Oocytes
lnjection of Xenopus oocytes with poly(A)+ mRNA isolated from different plants (maize, cucumber, and squash) results in the appearance of a voltageand time'dependent, potassium-selective, outward current that is similar to the outwardrectifying potassium current recorded in many higher plant cells. Maize shoots were found to be especially enriched in mRNA encoding such activity. A cDNA library ...
متن کاملXenopus oocytes
The induction of amphibian oocyte maturation with progesterone as well as the activation of sea urchin eggs at the time of fertilization result in increased protein synthesis. The increase in both cases involves the recruitment of maternal mRNA onto polysomes. Further, it has been reported that sea urchin eggs, like full-grown Xenopus oocytes, contain no spare translational capacity based on th...
متن کاملAntiporter in Xenopus Laevis Oocytes
The amiloride-sensitive Na + /H + antiporter in defolliculated oocytes of Xenopus laevis was characterized by measurements of H Na + influx and apparent H + efflux. Uptake of ^Na"" was linear over a 90-min incubation period and was inhibited approximately 80% with 5xlO~moll~ amiloride. Amiloride-sensitive sodium uptake was reduced following collagenase treatment or oocyte aging. Ko 5 for amilor...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1978
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.77.2.427